Rebecca L. Krisher, PhD
1. Paczkowski M, Schoolcraft, WB, Krisher RL. (2014) Fatty Acid Metabolism during maturation affects glucose uptake and is essential to oocyte competence. Reproduction 148:429-439.
2. Morbeck DE, Krisher RL, Herrick JH, Baumann NA, Matern D, Moyer T. (2014) Composition of commercial
media used for human embryo culture. Fertility and Sterility 102(3):759-766.
3. Herrick, JR, Strauss KJ, Schneiderman A, Rawlins M, Stevens J, Schoolcraft, WB, Krisher, RL. (2014) The beneficial effects of reduced magnesium during the oocyte to embryo transition are conserved in mice,
domestic cats and humans. Reproduction, Fertility and Development. https://dx.doi.org/10.1071/RD13268
4. Paczkowski, M, Silva, E., Schoolcraft, W.B., Krisher, R.L. (2013) Comparative importance of fatty acid β-oxidation to nuclear maturation, gene expression and glucose metabolism in mouse, bovine and porcine cumulus oocyte complexes. Biology of Reproduction. 88(5):111, 1-11. doi:10.1095/biolreprod.113.108548
5. Krisher, R.L. (2013) Oocyte and Embryo Metabolomics. Rodriguez-Martinez, H., Soede, N.M., Flowers, W.L. (eds.), Control of Pig Reproduction IX, Society of Reproduction and Fertility. 68:1-18.
6. Krisher, R.L. (2013) Oocyte Metabolism. In: Oocyte Physiology and Development in Domestic Animals. Ed:
R.L. Krisher. Wiley-Blackwell.
7. Krisher, R.L. (2013) In vivo and in vitro environmental effects on mammalian oocyte quality. Annual Reviews of Animal Biosciences. 1:6.1-6.25. Ed: H.A. Lewin, R.M Roberts. Linley Hall. doi:10.1146/annurev-animal-031412-103647.
8. Yuan, Y., Wheeler, M.B., Krisher, R.L. (2012) Disrupted Redox Homeostasis and Aberrant Redox Gene
Expression in Porcine Oocytes Contribute to Decreased Developmental Competence. Biology of Reproduction 87 (4) 78, 1-10; doi:10.1095/biolreprod.112.099952
9. Krisher, R.L., Prather, R.S. (2012) A role for the Warburg Effect in preimplantation embryo development: Metabolic modification to support rapid cell proliferation. Molecular Reproduction and Development. 79:311-320. DOI 10.1002/mrd.22037.
10. Silva, E., Paczkowski, M., Krisher, R.L. (2012) The effect of leptin on maturing porcine oocytes is dependent on glucose concentration. Molecular Reproduction and Development. 79(4): 296-307. Page 3
11. Krisher, R.L. (2012) Utility of Animal Models for Human Embryo Culture: Domestic Species. In: Embryo
Culture: Advances, Controversies, and Future Considerations. Eds: G. Smith, R. Pool, J. Swain. Methods in
Molecular Biology. 912:27-37. Humana Press.
12. Yuan, Y., Ida, J.M., Paczkowski, M., Krisher, R.L. (2011) Identification of developmental competence related genes in mature porcine oocytes. Molecular Reproduction and Development 78:565-575.
13. Paczkowski, M., Yuan, Y., Fleming-Waddell, J., Bidwell, C., Spurlock, D., Krisher, R.L. (2011) Alterations in the transcriptome of porcine oocytes derived from prepubertal and adult females is correlated with developmental potential. Journal of Animal Science 89:3561-3571.
14. Paczkowski, M., Krisher R.L. (2010) Aberrant Protein Expression is Associated with Decreased
Developmental Potential in Porcine Oocytes. Molecular Reproduction and Development 77:51-58. DOI
15. Herrick, J.R., Brad, A.M., Krisher, R.L. (2006) Chemical Manipulation of Glucose Metabolism in Porcine Oocytes: Effects on Nuclear and Cytoplasmic Maturation In Vitro. Reproduction 131: 289-298. DOI
16. Brad, A.M., Bormann, C.L., Swain, J.E., Durkin, R.E., Johnson, A.E., Clifford, A.L., Krisher, R.L. (2003) Glutathione and adenosine triphosphate content of in vivo and in vitro matured porcine oocytes. Molecular Reproduction and Development. 64:492-498.
17. Swain, J.E., Bormann, C.L., Clark, S.G., Walters, E.M., Wheeler, M.B., Krisher, R.L. (2002) Use of energy substrates by various stage preimplantation pig embryos produced in vivo and in vitro. Reproduction 123:253-260.
18. Gandhi, A.P., Lane, M., Gardner, D.K., Krisher, R.L. (2000) A single medium supports development of bovine embryos throughout maturation, fertilization and culture. Human Reproduction. 15:395-401.
19. Krisher, R.L., Lane, M., Bavister, B.D. (1999) Developmental competence and metabolism of bovine embryos cultured in semi-defined and defined culture media. Biology of Reproduction 60: 1345-1352.
20. Krisher R.L., Bavister, B.D. (1999) Enhanced glycolysis after maturation of bovine oocytes in vitro is associated with increased developmental competence. Molecular Reproduction and Development. 53:19-26.
Associate Editor, Reproduction, Fertility and Development, CSIRO Publishing. 2013-present. Editor, Oocyte Physiology and Development in Domestic Animals, Wiley Blackwell. Member, Comparative Medicine Review Committee of the NCRR, NIH. 2009-2012. Member, NIH special emphasis panel/scientific review group ZRG1 GGG-H; ZRG1 EMNR-D, ZDK1 GRB-J J2, ZRG1 PSE-B, and ZRG1 EMNR-S (chair); 2011, 2013, 2014.
Invited Speaker, 2014: American Embryo Transfer Association (Madison, WI), Havemeyer ‘Comparative Aspects of Reproductive Aging in the Mare and Woman’ Symposium (Estes Park, CO), WCRB Pre-Conference Symposium on Embryo Metabolism (Edinburgh, UK), and OvaScience Symposium ‘Embracing the Future of Fertility Technologies’ (Honolulu, HI).
Invited Speaker, 2013: Aspen/Snowmass Perinatal Biology Symposium (CO), and International Congress on Pig Reproduction, Poland.
Invited Speaker, 2011-2012: Utah State University, University of Nebraska-Lincoln, Colorado State University, University of Missouri-Columbia.
Invited Speaker, International Embryo Transfer Society, Annual meeting, 2010.
Invited speaker, Society for the Study of Reproduction Annual meeting, 2008, 2013.
Reproductive Biology Training Program, member, University of Illinois, Urbana-Champaign, 2007-2010.
Reviewer, NIH Special Emphasis Panel on Cryopreservation, NCRR. 2008.
Faculty Fellow, Committee on Institutional Cooperation (CIC) academic leadership program, 2006/07 Lead21 Fellow, 2005-2006
Member, Purdue Animal Care and Use Committee, 2005-2007
Purdue Diversity Action Team in Agriculture (DATA), 2003-2007; Co-chair, DATA committee Member, review panel USDA-NRI Animal Reproduction Program, 2000
Ad hoc reviewer, NIH, NSF, USDA, Burroughs Wellcome, ongoing
Reviewer, 8 scientific journals
Society for the Study of Reproduction; Member, Public Affairs, 2004-2006, Minority Affairs, 2008-2012, Program Committee 2010-2012; Awards Committee, 2014-present.
International Embryo Transfer Society; Program Co-Chair Annual Meeting, 2012; Board of Trustees, 2007-2010; Co-chair, CANDES Research committee, 2006-2010
American Society for Reproductive Medicine (ASRM); SBRT committee, secretary, 2014-present. North Central Regional Project 1038, Methods to increase reproductive efficiency in cattle; 1999-2010. Western Regional Project 1171, Germ cell and embryo development and manipulation for the improvement of livestock; 2006-2010.
The Honor Society of Phi Kappa Phi, 1994
Sigma Xi, The Scientific Research Honor Society; Secretary/Treasurer, 2005-2007
Gamma Sigma Delta, The Honor Society of Agriculture
Alpha Zeta Agricultural Honor Society
Krisher and Katz-Jaffe (PIs) Merck Serono, Grant for Fertility Innovation. 07/01/2013-06/30/2015. A novel strategy to improve live birth rates for women with diminished ovarian reserve (DOR).
Major Goals: Optimize the in vitro culture environment for the DOR oocyte, identify viable oocytes using cumulus cell biomarkers, select viable DOR embryos using time lapse microscopy.
Krisher (PI) Unisense FertiliTech, Inc. Equipment loan, Unisense EmbryoScope. 10/2013-2/2015. Developmental timing and embryo metabolism.
Major Goals: Define the association between developmental timing and embryo metabolism, optimize amino acids and growth factors for mouse embryo culture based upon these parameters.
Cupp (PI) USDA 2013-00853. 06/2013-05-2016. Causes and consequences of androgen excess on oocyte quality. RL Krisher, collaborator. Major Goals; Determine the role of gonadotropins on the granulosa cells to elevated androgen levels, and to compare follicular physiology and oocyte quality, both in cows with low and high granulosa cell steroidogenic efficiency.
Knox (PI) USDA (Integrated Solutions for Animal Agriculture). 01/01/2010-12/31/2013. Advancing Technology for Practical Use of Cryopreserved Boar Sperm to Improve Opportunities for Profitable Pork Production. Co-PIs: Miller, Krisher, Purdy, Rodriguez-Zas, Goldsmith, Clark, Stadler.
Major Goals: Investigate the fertility of cryopreserved boar semen.
Krisher (PI) Incept BioSystems, Inc. 12/01/2009-09/01/2010. Use of microfluidic devices for in vitro maturation of bovine oocytes.
Major Goals: To investigate the ability of a novel microfluidic system to support bovine oocyte maturation in vitro.
Krisher (PI) National Institutes of Health, NICHD. R21. 01/2009-12/2010. A Novel Model of Reproductive and Metabolic Features of Polycystic Ovary Syndrome.
Major Goals: To characterize the Ossabaw mini-pig as a model for Polycystic ovary syndrome (PCOS).
Krisher (PI) National Institutes of Health R03 4/2005-4/2007 Molecular Signature of Oocyte Developmental Competence.
Major Goals; To determine transcripts affected by female age and state of maturation that are associated with oocyte developmental competence using microarray technology. Role: PI
Krisher (PI) USDA-CSREES-NRICGP 8/2000 – 7/2003 Role of Oocyte Metabolic Activity in the Control of Porcine Embryo Development
Major Goals: To investigate the role of metabolism in the regulation of developmental competence of porcine oocytes, and to define parameters of energy substrates and metabolism that are correlated with increased developmental potential.
Krisher (PI) Genzyme Transgenics Corp. 8/2002 – 8/2004 Optimizing the in vitro maturation system for goat oocytes to support cytoplasmic maturation and increase subsequent embryonic development.
Major Goals: To develop a novel maturation medium that better supports cytoplasmic maturation and subsequent embryonic development for goats.