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Rebecca L. Krisher

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Rebecca L. Krisher 2017-05-27T06:44:01+00:00

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Rebecca L. Krisher, PhD

1. Paczkowski M, Schoolcraft, WB, Krisher RL. (2014) Fatty Acid Metabolism during maturation affects glucose uptake and is essential to oocyte competence. Reproduction 148:429-439.

2. Morbeck DE, Krisher RL, Herrick JH, Baumann NA, Matern D, Moyer T. (2014) Composition of commercial
media used for human embryo culture. Fertility and Sterility 102(3):759-766.

3. Herrick, JR, Strauss KJ, Schneiderman A, Rawlins M, Stevens J, Schoolcraft, WB, Krisher, RL. (2014) The beneficial effects of reduced magnesium during the oocyte to embryo transition are conserved in mice,
domestic cats and humans. Reproduction, Fertility and Development. https://dx.doi.org/10.1071/RD13268

4. Paczkowski, M, Silva, E., Schoolcraft, W.B., Krisher, R.L. (2013) Comparative importance of fatty acid β-oxidation to nuclear maturation, gene expression and glucose metabolism in mouse, bovine and porcine cumulus oocyte complexes. Biology of Reproduction. 88(5):111, 1-11. doi:10.1095/biolreprod.113.108548

5. Krisher, R.L. (2013) Oocyte and Embryo Metabolomics. Rodriguez-Martinez, H., Soede, N.M., Flowers, W.L. (eds.), Control of Pig Reproduction IX, Society of Reproduction and Fertility. 68:1-18.

6. Krisher, R.L. (2013) Oocyte Metabolism. In: Oocyte Physiology and Development in Domestic Animals. Ed:
R.L. Krisher. Wiley-Blackwell.

7. Krisher, R.L. (2013) In vivo and in vitro environmental effects on mammalian oocyte quality. Annual Reviews of Animal Biosciences. 1:6.1-6.25. Ed: H.A. Lewin, R.M Roberts. Linley Hall. doi:10.1146/annurev-animal-031412-103647.

8. Yuan, Y., Wheeler, M.B., Krisher, R.L. (2012) Disrupted Redox Homeostasis and Aberrant Redox Gene
Expression in Porcine Oocytes Contribute to Decreased Developmental Competence. Biology of Reproduction 87 (4) 78, 1-10; doi:10.1095/biolreprod.112.099952

9. Krisher, R.L., Prather, R.S. (2012) A role for the Warburg Effect in preimplantation embryo development: Metabolic modification to support rapid cell proliferation. Molecular Reproduction and Development. 79:311-320. DOI 10.1002/mrd.22037.

10. Silva, E., Paczkowski, M., Krisher, R.L. (2012) The effect of leptin on maturing porcine oocytes is dependent on glucose concentration. Molecular Reproduction and Development. 79(4): 296-307. Page 3

11. Krisher, R.L. (2012) Utility of Animal Models for Human Embryo Culture: Domestic Species. In: Embryo
Culture: Advances, Controversies, and Future Considerations. Eds: G. Smith, R. Pool, J. Swain. Methods in
Molecular Biology. 912:27-37. Humana Press.

12. Yuan, Y., Ida, J.M., Paczkowski, M., Krisher, R.L. (2011) Identification of developmental competence related genes in mature porcine oocytes. Molecular Reproduction and Development 78:565-575.

13. Paczkowski, M., Yuan, Y., Fleming-Waddell, J., Bidwell, C., Spurlock, D., Krisher, R.L. (2011) Alterations in the transcriptome of porcine oocytes derived from prepubertal and adult females is correlated with developmental potential. Journal of Animal Science 89:3561-3571.

14. Paczkowski, M., Krisher R.L. (2010) Aberrant Protein Expression is Associated with Decreased
Developmental Potential in Porcine Oocytes. Molecular Reproduction and Development 77:51-58. DOI
10.1002/mrd.21102.

15. Herrick, J.R., Brad, A.M., Krisher, R.L. (2006) Chemical Manipulation of Glucose Metabolism in Porcine Oocytes: Effects on Nuclear and Cytoplasmic Maturation In Vitro. Reproduction 131: 289-298. DOI
10.1530/rep.1.00835.

16. Brad, A.M., Bormann, C.L., Swain, J.E., Durkin, R.E., Johnson, A.E., Clifford, A.L., Krisher, R.L. (2003) Glutathione and adenosine triphosphate content of in vivo and in vitro matured porcine oocytes. Molecular Reproduction and Development. 64:492-498.

17. Swain, J.E., Bormann, C.L., Clark, S.G., Walters, E.M., Wheeler, M.B., Krisher, R.L. (2002) Use of energy substrates by various stage preimplantation pig embryos produced in vivo and in vitro. Reproduction 123:253-260.

18. Gandhi, A.P., Lane, M., Gardner, D.K., Krisher, R.L. (2000) A single medium supports development of bovine embryos throughout maturation, fertilization and culture. Human Reproduction. 15:395-401.

19. Krisher, R.L., Lane, M., Bavister, B.D. (1999) Developmental competence and metabolism of bovine embryos cultured in semi-defined and defined culture media. Biology of Reproduction 60: 1345-1352.

20. Krisher R.L., Bavister, B.D. (1999) Enhanced glycolysis after maturation of bovine oocytes in vitro is associated with increased developmental competence. Molecular Reproduction and Development. 53:19-26.

Associate Editor, Reproduction, Fertility and Development, CSIRO Publishing. 2013-present. Editor, Oocyte Physiology and Development in Domestic Animals, Wiley Blackwell. Member, Comparative Medicine Review Committee of the NCRR, NIH. 2009-2012. Member, NIH special emphasis panel/scientific review group ZRG1 GGG-H; ZRG1 EMNR-D, ZDK1 GRB-J J2, ZRG1 PSE-B, and ZRG1 EMNR-S (chair); 2011, 2013, 2014.

Invited Speaker, 2014: American Embryo Transfer Association (Madison, WI), Havemeyer ‘Comparative Aspects of Reproductive Aging in the Mare and Woman’ Symposium (Estes Park, CO), WCRB Pre-Conference Symposium on Embryo Metabolism (Edinburgh, UK), and OvaScience Symposium ‘Embracing the Future of Fertility Technologies’ (Honolulu, HI).

Invited Speaker, 2013: Aspen/Snowmass Perinatal Biology Symposium (CO), and International Congress on Pig Reproduction, Poland.

Invited Speaker, 2011-2012: Utah State University, University of Nebraska-Lincoln, Colorado State University, University of Missouri-Columbia.

Invited Speaker, International Embryo Transfer Society, Annual meeting, 2010.

Invited speaker, Society for the Study of Reproduction Annual meeting, 2008, 2013.

Reproductive Biology Training Program, member, University of Illinois, Urbana-Champaign, 2007-2010.

Reviewer, NIH Special Emphasis Panel on Cryopreservation, NCRR. 2008.

Faculty Fellow, Committee on Institutional Cooperation (CIC) academic leadership program, 2006/07 Lead21 Fellow, 2005-2006

Member, Purdue Animal Care and Use Committee, 2005-2007

Purdue Diversity Action Team in Agriculture (DATA), 2003-2007; Co-chair, DATA committee Member, review panel USDA-NRI Animal Reproduction Program, 2000

Ad hoc reviewer, NIH, NSF, USDA, Burroughs Wellcome, ongoing

Reviewer, 8 scientific journals

Society for the Study of Reproduction; Member, Public Affairs, 2004-2006, Minority Affairs, 2008-2012, Program Committee 2010-2012; Awards Committee, 2014-present.

International Embryo Transfer Society; Program Co-Chair Annual Meeting, 2012; Board of Trustees, 2007-2010; Co-chair, CANDES Research committee, 2006-2010

American Society for Reproductive Medicine (ASRM); SBRT committee, secretary, 2014-present. North Central Regional Project 1038, Methods to increase reproductive efficiency in cattle; 1999-2010. Western Regional Project 1171, Germ cell and embryo development and manipulation for the improvement of livestock; 2006-2010.

The Honor Society of Phi Kappa Phi, 1994

Sigma Xi, The Scientific Research Honor Society; Secretary/Treasurer, 2005-2007

Gamma Sigma Delta, The Honor Society of Agriculture

Alpha Zeta Agricultural Honor Society

ONGOING SUPPORT

Krisher and Katz-Jaffe (PIs) Merck Serono, Grant for Fertility Innovation. 07/01/2013-06/30/2015. A novel strategy to improve live birth rates for women with diminished ovarian reserve (DOR).

Major Goals: Optimize the in vitro culture environment for the DOR oocyte, identify viable oocytes using cumulus cell biomarkers, select viable DOR embryos using time lapse microscopy.


Krisher (PI) Unisense FertiliTech, Inc. Equipment loan, Unisense EmbryoScope. 10/2013-2/2015. Developmental timing and embryo metabolism.

Major Goals: Define the association between developmental timing and embryo metabolism, optimize amino acids and growth factors for mouse embryo culture based upon these parameters.


Cupp (PI) USDA 2013-00853. 06/2013-05-2016. Causes and consequences of androgen excess on oocyte quality. RL Krisher, collaborator. Major Goals; Determine the role of gonadotropins on the granulosa cells to elevated androgen levels, and to compare follicular physiology and oocyte quality, both in cows with low and high granulosa cell steroidogenic efficiency.


COMPLETED SUPPORT

Knox (PI) USDA (Integrated Solutions for Animal Agriculture). 01/01/2010-12/31/2013. Advancing Technology for Practical Use of Cryopreserved Boar Sperm to Improve Opportunities for Profitable Pork Production. Co-PIs: Miller, Krisher, Purdy, Rodriguez-Zas, Goldsmith, Clark, Stadler.

Major Goals: Investigate the fertility of cryopreserved boar semen.


Krisher (PI) Incept BioSystems, Inc. 12/01/2009-09/01/2010. Use of microfluidic devices for in vitro maturation of bovine oocytes.

Major Goals: To investigate the ability of a novel microfluidic system to support bovine oocyte maturation in vitro.


Krisher (PI) National Institutes of Health, NICHD. R21. 01/2009-12/2010. A Novel Model of Reproductive and Metabolic Features of Polycystic Ovary Syndrome.

Major Goals: To characterize the Ossabaw mini-pig as a model for Polycystic ovary syndrome (PCOS).


Krisher (PI) National Institutes of Health R03 4/2005-4/2007 Molecular Signature of Oocyte Developmental Competence.

Major Goals; To determine transcripts affected by female age and state of maturation that are associated with oocyte developmental competence using microarray technology. Role: PI


Krisher (PI) USDA-CSREES-NRICGP 8/2000 – 7/2003 Role of Oocyte Metabolic Activity in the Control of Porcine Embryo Development

Major Goals: To investigate the role of metabolism in the regulation of developmental competence of porcine oocytes, and to define parameters of energy substrates and metabolism that are correlated with increased developmental potential.


Krisher (PI) Genzyme Transgenics Corp. 8/2002 – 8/2004 Optimizing the in vitro maturation system for goat oocytes to support cytoplasmic maturation and increase subsequent embryonic development.

Major Goals: To develop a novel maturation medium that better supports cytoplasmic maturation and subsequent embryonic development for goats.

Research Staff

 Nicolas Santiquet
Nicolas SantiquetPost Doctoral Researcher
Nicolas grew up in France. He obtained his bachelor degree in 2005 followed by a Master degree in cellular and integrative biology at the University of Grenoble (France) in 2006. He continued his education in Canada at the University Laval in Quebec City where he obtained a Master degree in Animal Science and a PhD in Physiology-Endocrinology. During his master degree, he focused on the involvement of bone marrow stem cells in the post natal renewal of adult oocytes in mice and bovine. His PhD focused on the regulation of gap junctional communication during in vitro maturation of cumulus oocyte complexes. In January 2014, Nicolas joined CSU as a postdoctoral scientist and is collaborating with CCRM to continue his work in the reproductive field. In his free time Nicolas enjoys playing soccer and snowboarding in the Colorado Rockies. [PUBLICATIONS]
Jason Herrick
Jason HerrickSenior Scientist
Jason received his B.S. from Michigan State University in 1996, his M.S. in Zoology from Ohio State University in 1998, and his Ph.D. in 2003 from Purdue University studying in vitro oocyte maturation in pigs and goats. In 2004, Jason joined the Center for Conservation and Research of Endangered Wildlife at the Cincinnati Zoo and Botanical Garden as a post-doctoral fellow and conducted research on the physiology of feline embryos. He was an assistant professor at the University of Illinois in the Dept. of Comparative Biosciences from 2007 to 2011 and started work at CCRM in June 2011. His primary research interest is how the composition of the maternal diet or the culture medium affects cellular metabolism in the embryo and the ability of the embryo to develop successfully. Jason also has a strong interest in wildlife conservation and conducts research to develop assisted reproductive technologies for endangered species. [PUBLICATIONS]
Elena Silva
Elena SilvaPost Doctoral Researcher
Elena received a M.S. in Dairy Science from University of Wisconsin where she studied estrus synchronization protocols in dairy cows and her PhD from University of Illinois studying the impact of increased level of leptin hormone on embryo metabolism of glucose. Currently, as a post-doctoral fellow at the National Foundation for Fertility Research, her research projects are centered on the impact of maternal aging on embryo function. The goal is to develop a specific culture system targeting embryo’s requirements during growth aiming to reduce the rates of aneuploidy and improve overall embryonic competence. The current application will lay the foundation to develop clinical approaches for assisted reproductive technology. [PUBLICATIONS]
Alison Ermisch
Alison ErmischResearch Embryologist
Alison graduated from California Polytechnic State University, San Luis Obispo in 2013, where she received her Bachelor’s degree in Animal Science with an emphasis in animal reproduction and biology. She moved from California to join the CCRM research group after completing a research fellowship at the San Diego Zoo Institute for Conservation Research in the Reproductive Physiology division. Her current research projects involve exploring the effects of nutrient concentrations in culture media on embryo development and quality, as well as examining the impact of culture volume in a single embryo time-lapse system. She also performs non-surgical embryo transfers to assess embryo viability. In her free time, Alison enjoys being outdoors and exploring her new home state of Colorado.
Jack Becker
Jack BeckerResearch Laboratory Assistant
Jack graduated from the University of Colorado at Boulder in 2015, receiving a bachelor’s in Molecular, Cellular, and Developmental Biology with an emphasis in Molecular Neurobiology. He was previously at the University of Colorado Anschutz Medical Campus investigating the molecular signals involved in Neuronal Crest Cell patterning during development. At CU he was also involved with studying metabolic gene expression in Burmese Pythons before and after feeding, with the intent to better understand how pythons increase heart growth after enormous meals; something that would lead to certain cardiovascular diseases in humans. He began his career at Fertility Labs of Colorado in 2016, working as a Research Laboratory Assistant for Rebecca Krisher. Outside of work Jack enjoys watching the local professional sports teams and partaking in the abundant outdoor activities in Colorado.
Rolando Pasquariello
Rolando PasquarielloPost-Doctoral Fellow
Rolando got his M.D in Biotechnology in medicine at the Second University of Naples (Italy) discussing his thesis on the analysis of DNA methylation patterns of patients with Silver-Russel syndrome. Then, he received his PhD from University of Milan (Italy) studying microRNAs related to gametes and early embryos. Recently, Rolando moved to USA to start a postdoctoral fellowship with Colorado State University and is collaborating with Colorado Center for Reproductive Medicine working on oocyte and embryo metabolisms. The main objective is to elucidate the role of microRNA in controlling signaling pathways critical for preimplantation embryo development in mouse and cattle. During his free time, Rolando enjoys outdoor activities and cooking new recipes combining American and Italian tastes. [PUBLICATIONS]
Andrew Leach
Andrew LeachSummer Intern
Andrew is currently pursuing a bachelor’s degree in Biochemical Engineering at the Colorado School of Mines. While at Mines, Andrew investigated the effects of knocking out two genes, desaturase B and transaldolase C, in Cyanobacteria. This was accomplished through the use of Gibson Assembly with restriction enzymes, a plasmid backbone, and genomic DNA for the gene of interest. Currently, Andrew is working towards designing an improved culture dish for in vitro embryonic development. This is being investigated though 3-D printing technology combined with an experimental polymer as the working conductive filament. In his free time, Andrew enjoys experiencing the outdoors, swimming, and eating burritos smothered in green chili.
Becca Kile
Becca graduated in 2015 from California Polytechnic State University, San Luis Obispo where she received a Bachelor’s degree in Animal Science with an emphasis in poultry production and pre-veterinarian medicine. Then she received her Master’s degree in Biomedical Sciences, specializing in assisted reproductive technologies from Colorado State University, Fort Collins in 2016. Before joining CCRM, Becca completed an internship at Boston IVF in Waltham, MA where she tried to develop a protocol for the cryopreservation of oligospermic semen using low volumes in order to facilitate it’s preparation for in vitro fertilization. Additionally, she examined if there was an association between blastocyst grade and chromosomal abnormalities during days 5 and 6 of in vitro embryo culture. In her free time, Becca enjoys traveling with her Yorkshire Terrier, “Optimus Prime”, and discovering great food in her new home in the Denver metro area.